4 words for RU 1–2 letters

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1 letter (2)

r u
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2 letters (2)

ru ur
Poet’s word, with inverted comma, for the end of the day
?
E
?
E
?
N
Hint 1 meaning
The old plural of Eye.
Hint 2 anagram
ENE
Hint 3 another clue
AND STILL ___
27 +1