4 words for PU 1–2 letters

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1 letter (2)

p u

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Alphabet (Z–A)

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2 letters (2)

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Poet’s word, with inverted comma, for the end of the day

?

E

?

E

?

N

Hint 1 meaning

The old plural of Eye.

Hint 2 anagram

EEN

Hint 3 another clue

AND STILL ___

20 +1